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basic fibroblast growth factor bfgf  (Miltenyi Biotec)


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    Miltenyi Biotec basic fibroblast growth factor bfgf
    Basic Fibroblast Growth Factor Bfgf, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+fgf+2/Human+FGF-2%2C+premium+grade/pm42336896-211-24-29
    Average 95 stars, based on 149 article reviews
    basic fibroblast growth factor bfgf - by Bioz Stars, 2026-10
    95/100 stars

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    Cell Culture:

    Article Title: Effects of Magnesium-Doped Hydroxyapatite Nanoparticles on Bioink Formulation for Bone Tissue Engineering.
    Article Snippet: .. Cells were cultured and expanded in CellStack (Nunc, Thermo Fisher Scientific) to reach the desired number of cells, under humidified 5% CO2 at 37 °C in basal media (BM) composed of DMEM with 10% fetal bovine serum (FBS; HyClone, GE Healthcare, Utah, U.S.A.),1% penicillin−streptomycin (P/S; 10 000 U/mL, Thermo Fisher Scientific, Gibco) (basal medium; BM) and supplemented with 5 ng/mL human FGF-2 (Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Effects of Magnesium-Doped Hydroxyapatite Nanoparticles on Bioink Formulation for Bone Tissue Engineering
    Article Snippet: .. Cells were cultured and expanded in CellStack (Nunc, Thermo Fisher Scientific) to reach the desired number of cells, under humidified 5% CO 2 at 37 °C in basal media (BM) composed of DMEM with 10% fetal bovine serum (FBS; HyClone, GE Healthcare, Utah, U.S.A.),1% penicillin–streptomycin (P/S; 10 000 U/mL, Thermo Fisher Scientific, Gibco) (basal medium; BM) and supplemented with 5 ng/mL human FGF-2 (Miltenyi Biotec, Bergisch Gladbach, Germany). .. To prepare the biomaterial inks for cross-linking, sterile-filtered lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, >98%, TCI, Tokyo, Japan) was added (stock solution 10 mg/mL), and the whole solution was mixed for 1 h at 37 °C in the dark, covered to avoid evaporation, reaching the final LAP concentration of 0.1% w/ w tot .

    Article Title: A Human Neuronal Co-Culture System Reveals Early Tumor-Neuron Communication and Targetable Pathways in Glioblastoma
    Article Snippet: .. NU-757 cells were cultured under adherent, serum-free conditions in phenol red–free Neurobasal medium (Gibco, Cat. #12348017) supplemented with B-27 Supplement minus Vitamin A (Gibco, Cat. #12587010), human FGF-2 (10 ng/mL; Miltenyi Biotec, Cat. #130-097-751), human EGF (10 ng/mL; Miltenyi Biotec, Cat. #130-093-842), GlutaMAX (1×; Gibco, Cat. #35050061), sodium pyruvate (1 mM; Gibco, Cat. #11360070), and penicillin/streptomycin (100 U/mL and 100 μg/mL, respectively; Gibco, Cat. #15070063). .. NU-757 cells were seeded onto tissue culture plates pre-coated with Geltrex LDEV-free stem cell, a qualified reduced-growth factor basement membrane matrix (Life Technologies, Cat. #A1413302).

    Article Title: Investigating the Potential of Multilineage Differentiating Stress-Enduring Cells for Osteochondral Healing.
    Article Snippet: .. MSCs after VA labeling were cultured at 37°C with 5% CO2 in low-glucose Dulbecco’s minimum essential medium (DMEM) supplied with 10% fetal bovine serum (FBS; HyClone, Logan, UT), 1 ng/ml human FGF-2 (Miltenyi Biotec, Bergisch Gladbach, Germany), 0.1 mg/ml kanamycin (Invitrogen, Carlsbad, CA), and 1% GlutaMAX (Life Technologies, Carlsbad, CA). .. After 90% confluence was reached, the cells were subcultured at a ratio of 1:2 with 0.25% trypsin-EDTA (Life Technologies, Carlsbad, CA).

    Article Title: Optimizing gelatin methacryloyl-based bioinks with nanohydroxyapatite for 3D bioprinting in bone tissue engineering
    Article Snippet: In recent years, the incorporation of a ceramic phase such as nanohydroxyapatite (nHA) into gelatin methacryloyl (GelMA)-based bioinks has gained attention for extrusion-based bioprinting to replicate the inorganic mineral phase of bone tissue.. However, the effects of different nHA concentrations in GelMA bioinks on printability, shape fidelity, long-term stability, cell viability, and osteogenic activity have not been fully investigated.. Therefore, the current study investigates the incorporation of different concentrations of nHA (0, 1, 3 and 5% w/ v) into GelMA/gelatin/nanocellulose bioink for printing human bone marrow-derived stromal cells for bone tissue engineering applications.

    Article Title: Neuropilin-1-peptide based antibody, highlighting new epitope associated to glycosaminoglycan-modified neuropilin-1 and methods of use thereof
    Article Snippet: The analysis was carried out using Isis software (MetaSystems, version 3.9.1) as described previously. .. Micronucleus assay Circulating tumor cells, CTC41 and CTC41.5E were cultured in RPMI 1640 Medium GlutaMAXTM (61/870,036, Gibco) supplemented with human EGF, premium (130-097-749, Miltenyi), human FGF-2, premium (130-093-564, Miltenyi), INSULIN-TRANS-SEL-A (51300044, Gibco) 10% inactivated human serum (H4522, Sigma). ..

    Article Title: Systematic development and bioprinting of novel nanostructured multi-material bioinks for bone tissue engineering.
    Article Snippet: .. BMSC 1 was used in all experiments, excluding Alkaline phosphatase staining which was executed using BMSC2.During cellmaintenance, but not during experimentation, the cells were cultured in a medium of 5% (v/v) human serum (HSBW; S4190-100, AB Male HIV tested, Biowest, Nuaillé, France) in MEM Alpha Medium (1X, 22 571-020, GibcoTM, Thermo Fisher Scientific) with 1% P/S (v/v), supplemented with 5 ng ml−1 Human FGF-2 (130-093-838, Miltenyi Biotec, Bergisch Gladbach, Germany) to maintain the multipotency of the hBMSCs. ..

    Article Title: Investigating the Potential of Multilineage Differentiating Stress-Enduring Cells for Osteochondral Healing
    Article Snippet: .. MSCs after VA labeling were cultured at 37°C with 5% CO 2 in low-glucose Dulbecco’s minimum essential medium (DMEM) supplied with 10% fetal bovine serum (FBS; HyClone, Logan, UT), 1 ng/ml human FGF-2 (Miltenyi Biotec, Bergisch Gladbach, Germany), 0.1 mg/ml kanamycin (Invitrogen, Carlsbad, CA), and 1% GlutaMAX (Life Technologies, Carlsbad, CA). .. After 90% confluence was reached, the cells were subcultured at a ratio of 1:2 with 0.25% trypsin-EDTA (Life Technologies, Carlsbad, CA).

    Labeling:

    Article Title: Investigating the Potential of Multilineage Differentiating Stress-Enduring Cells for Osteochondral Healing.
    Article Snippet: .. MSCs after VA labeling were cultured at 37°C with 5% CO2 in low-glucose Dulbecco’s minimum essential medium (DMEM) supplied with 10% fetal bovine serum (FBS; HyClone, Logan, UT), 1 ng/ml human FGF-2 (Miltenyi Biotec, Bergisch Gladbach, Germany), 0.1 mg/ml kanamycin (Invitrogen, Carlsbad, CA), and 1% GlutaMAX (Life Technologies, Carlsbad, CA). .. After 90% confluence was reached, the cells were subcultured at a ratio of 1:2 with 0.25% trypsin-EDTA (Life Technologies, Carlsbad, CA).

    Article Title: Investigating the Potential of Multilineage Differentiating Stress-Enduring Cells for Osteochondral Healing
    Article Snippet: .. MSCs after VA labeling were cultured at 37°C with 5% CO 2 in low-glucose Dulbecco’s minimum essential medium (DMEM) supplied with 10% fetal bovine serum (FBS; HyClone, Logan, UT), 1 ng/ml human FGF-2 (Miltenyi Biotec, Bergisch Gladbach, Germany), 0.1 mg/ml kanamycin (Invitrogen, Carlsbad, CA), and 1% GlutaMAX (Life Technologies, Carlsbad, CA). .. After 90% confluence was reached, the cells were subcultured at a ratio of 1:2 with 0.25% trypsin-EDTA (Life Technologies, Carlsbad, CA).

    Staining:

    Article Title: Systematic development and bioprinting of novel nanostructured multi-material bioinks for bone tissue engineering.
    Article Snippet: .. BMSC 1 was used in all experiments, excluding Alkaline phosphatase staining which was executed using BMSC2.During cellmaintenance, but not during experimentation, the cells were cultured in a medium of 5% (v/v) human serum (HSBW; S4190-100, AB Male HIV tested, Biowest, Nuaillé, France) in MEM Alpha Medium (1X, 22 571-020, GibcoTM, Thermo Fisher Scientific) with 1% P/S (v/v), supplemented with 5 ng ml−1 Human FGF-2 (130-093-838, Miltenyi Biotec, Bergisch Gladbach, Germany) to maintain the multipotency of the hBMSCs. ..



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